Tools for fragment based drug discovery

SPR Validation

< Validation & Characterization

SPR VALIDATION


SPR is a powerful, industry accepted ligand discovery technology that is orthogonal to NMR. We have shown that the combination of TINS screening with Biacore™ fragment characterization forms a powerful workflow for drug discovery (Kobayashi, J. Biomol. Screen., 2010). Our integrated platform of TINS and SPR (Biacore™ T200 & 4000) allows us to further leverage our expertise in protein immobilization for the benefit of our customers.

Biacore_T200

The sensorgrams above show the titrations of three Hsp90 fragment hits that were identified by TINS screening. The KD determined by Biacore™, varying from double digit µM to a few hundred µM, is in good correspondence with the values determined via protein observed NMR, as depicted in the figure below.

SPR_HSQC_corr


< Validation & Characterization