LIGAND VALIDATION & CHARACTERIZATION
Surface Plasmon Resonance (SPR) is a powerful, industry accepted ligand discovery technology that is orthogonal to NMR. In particular, TINS and SPR are highly complementary since both require target immobilization, while TINS provides high sensitivity to weak interactions and SPR is highly quantitative. ZoBio has a unique, highly integrated platform of TINS and and two Biacore™ instruments (T200 & 4000, respectively) that allows us to further leverage our unparalleled expertise in protein immobilization for the benefit of our customers.
COMPETITION BINDING WITH NMR
One powerful method of validating hits is to perform a competition binding assay using NMR / TINS. Here, known ligands (inhibitors) are used to validate the binding of new fragments. When performing a competition study in TINS, each hit is reassayed as a singleton, thereby providing confirmation of binding, and subsequently in the presence of saturating amounts of a competitor, providing binding site information.