Tools for fragment based drug discovery

Structural Biology

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STRUCTURAL BIOLOGY


It is widely recognized that the process of elaborating a fragment hit towards a lead is rendered much more efficient by the availability of structural information on the ligand – target interaction. We provide an array of technologies to obtain structural information on ligand – protein complexes, including challenging targets.

The ZoBio NMR Pyramid® illustrates the different levels of structural information on ligand – target complexes that can be obtained by NMR. At the bottom of the NMR Pyramid, Competition Binding experiments provide low resolution structural information on the binding location of ligands with minimal requirements to the protein. Moving upwards through the Pyramid, isotope labeled protein is required and increased NMR efforts are needed, which leads to higher resolution structural information.

Structure Elucidation   /   Binding Orientation   /   Binding Site Location   /   Competition Binding

NMR_Pyramid

Click on the NMR Pyramid for a description of the different stages of the NMR Pyramid, starting with Competition Binding experiments and moving up to full NOE-based Structure Elucidation by NMR.


< Hit DiscoveryHit to Lead Development >